Long-term Rasamsonia argillacea complex species colonization revealed by rep-PCR in cystic fibrosis patients.
Identifieur interne : 000050 ( France/Analysis ); précédent : 000049; suivant : 000051Long-term Rasamsonia argillacea complex species colonization revealed by rep-PCR in cystic fibrosis patients.
Auteurs : Abdelmounaim Mouhajir [Maroc] ; Olivier Matray [France] ; Sandrine Giraud [France] ; Laurent Mély [France] ; Christophe Marguet [France] ; Isabelle Sermet-Gaudelus [France] ; Solène Le Gal [France] ; Franck Labbé [France] ; Christine Person [France] ; Françoise Troussier [France] ; Jean Jacques Ballet [France] ; Gilles Gargala [France] ; Rachid Zouhair [Maroc] ; Marie-Elisabeth Bougnoux [France] ; Jean-Philippe Bouchara [France] ; Loïc Favennec [France]Source :
- Journal of clinical microbiology [ 1098-660X ] ; 2016.
Abstract
The aim of this work was to document molecular epidemiology of Rasamsonia argillacea species complex isolates from cystic fibrosis (CF) patients. In this work, 116 isolates belonging to this species complex and collected from 26 CF patients and one patient with chronic granulomatous disease were characterized using PCR amplification assays of repetitive DNA sequences, and electrophoretic separation of amplicons (rep-PCR). Data revealed a clustering consistent with molecular species identification. A single species was recovered from most patients. Rasamsonia aegroticola was the most common species, followed by R. argillacea stricto sensu and R. piperina, while R. eburnea was not identified. Of 29 genotypes, 7 were shared by distinct patients, while 22 were patient-specific. In each clinical sample, most isolates exhibited an identical genotype. Genotyping of isolates recovered from sequential samples from the same patient confirmed the capability of R. aegroticola and R. argillacea isolates to chronically colonize the airways. A unique genotype was recovered from two siblings during a 6-month period. In the other cases, a largely dominant genotype was detected. Present results which support the use of rep-PCR for both identification and genotyping for the R. argillacea species complex, provide the first molecular evidence of chronic airway colonization by these fungi in CF patients.
DOI: 10.1128/JCM.01462-16
PubMed: 27605712
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 000015
- to stream PubMed, to step Curation: 000015
- to stream PubMed, to step Checkpoint: 000015
- to stream Ncbi, to step Merge: 000565
- to stream Ncbi, to step Curation: 000565
- to stream Ncbi, to step Checkpoint: 000565
- to stream Main, to step Merge: 000050
- to stream Main, to step Curation: 000051
- to stream Main, to step Exploration: 000051
- to stream France, to step Extraction: 000050
Links to Exploration step
pubmed:27605712Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Long-term Rasamsonia argillacea complex species colonization revealed by rep-PCR in cystic fibrosis patients.</title><author><name sortKey="Mouhajir, Abdelmounaim" sort="Mouhajir, Abdelmounaim" uniqKey="Mouhajir A" first="Abdelmounaim" last="Mouhajir">Abdelmounaim Mouhajir</name><affiliation wicri:level="1"><nlm:affiliation>L'UNAM Université, Université d'Angers, Groupe d'Etude des Interactions Hôte-Pathogène, EA 3142, Angers, France University Moulay Ismail, Faculty of Sciences, Meknes, Morocco.</nlm:affiliation><country xml:lang="fr">Maroc</country><wicri:regionArea>L'UNAM Université, Université d'Angers, Groupe d'Etude des Interactions Hôte-Pathogène, EA 3142, Angers, France University Moulay Ismail, Faculty of Sciences, Meknes</wicri:regionArea><wicri:noRegion>Meknes</wicri:noRegion></affiliation></author><author><name sortKey="Matray, Olivier" sort="Matray, Olivier" uniqKey="Matray O" first="Olivier" last="Matray">Olivier Matray</name><affiliation wicri:level="3"><nlm:affiliation>Laboratoire de Parasitologie-Mycologie, Centre Hospitalier Universitaire, Rouen, France Normandie Univ, UNIROUEN, EA 3800, 76000 Rouen, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Parasitologie-Mycologie, Centre Hospitalier Universitaire, Rouen, France Normandie Univ, UNIROUEN, EA 3800, 76000 Rouen</wicri:regionArea><placeName><region type="region" nuts="2">Région Normandie</region><region type="old region" nuts="2">Haute-Normandie</region><settlement type="city">Rouen</settlement></placeName></affiliation></author><author><name sortKey="Giraud, Sandrine" sort="Giraud, Sandrine" uniqKey="Giraud S" first="Sandrine" last="Giraud">Sandrine Giraud</name><affiliation wicri:level="1"><nlm:affiliation>L'UNAM Université, Université d'Angers, Groupe d'Etude des Interactions Hôte-Pathogène, EA 3142, Angers, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>L'UNAM Université, Université d'Angers, Groupe d'Etude des Interactions Hôte-Pathogène, EA 3142, Angers</wicri:regionArea><placeName><settlement type="city">Angers</settlement></placeName></affiliation></author><author><name sortKey="Mely, Laurent" sort="Mely, Laurent" uniqKey="Mely L" first="Laurent" last="Mély">Laurent Mély</name><affiliation wicri:level="1"><nlm:affiliation>Centre de Ressources et de Compétences en Mucoviscidose, Hôpital Renée Sabran, Giens, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Centre de Ressources et de Compétences en Mucoviscidose, Hôpital Renée Sabran, Giens</wicri:regionArea><wicri:noRegion>Giens</wicri:noRegion><wicri:noRegion>Giens</wicri:noRegion></affiliation></author><author><name sortKey="Marguet, Christophe" sort="Marguet, Christophe" uniqKey="Marguet C" first="Christophe" last="Marguet">Christophe Marguet</name><affiliation wicri:level="1"><nlm:affiliation>Centre de Ressources et de Compétences en Mucoviscidose, Service de Pédiatrie, Hôpital Charles Nicolle, Rouen, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Centre de Ressources et de Compétences en Mucoviscidose, Service de Pédiatrie, Hôpital Charles Nicolle, Rouen</wicri:regionArea><placeName><settlement type="city">Rouen</settlement></placeName></affiliation></author><author><name sortKey="Sermet Gaudelus, Isabelle" sort="Sermet Gaudelus, Isabelle" uniqKey="Sermet Gaudelus I" first="Isabelle" last="Sermet-Gaudelus">Isabelle Sermet-Gaudelus</name><affiliation wicri:level="1"><nlm:affiliation>Centre de Ressources et de Compétences en Mucoviscidose, Unité de Pédiatrie, Hôpital Necker-Enfants Malades, Paris, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Centre de Ressources et de Compétences en Mucoviscidose, Unité de Pédiatrie, Hôpital Necker-Enfants Malades, Paris</wicri:regionArea><placeName><settlement type="city">Paris</settlement></placeName></affiliation></author><author><name sortKey="Le Gal, Solene" sort="Le Gal, Solene" uniqKey="Le Gal S" first="Solène" last="Le Gal">Solène Le Gal</name><affiliation wicri:level="1"><nlm:affiliation>L'UNAM Université, Université d'Angers, Groupe d'Etude des Interactions Hôte-Pathogène, EA 3142, Angers, France Laboratoire de Parasitologie-Mycologie, Centre Hospitalier Universitaire, Brest, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>L'UNAM Université, Université d'Angers, Groupe d'Etude des Interactions Hôte-Pathogène, EA 3142, Angers, France Laboratoire de Parasitologie-Mycologie, Centre Hospitalier Universitaire, Brest</wicri:regionArea><placeName><settlement type="city">Brest</settlement></placeName></affiliation></author><author><name sortKey="Labbe, Franck" sort="Labbe, Franck" uniqKey="Labbe F" first="Franck" last="Labbé">Franck Labbé</name><affiliation wicri:level="1"><nlm:affiliation>Laboratoire de Microbiologie, Hôpital Jacques Monod, Le Havre, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Microbiologie, Hôpital Jacques Monod, Le Havre</wicri:regionArea><placeName><settlement type="city">Le Havre</settlement></placeName></affiliation></author><author><name sortKey="Person, Christine" sort="Person, Christine" uniqKey="Person C" first="Christine" last="Person">Christine Person</name><affiliation wicri:level="1"><nlm:affiliation>Centre de Ressources et de Compétences en Mucoviscidose Adultes, Service de Pneumologie, Centre Hospitalier Universitaire, Angers, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Centre de Ressources et de Compétences en Mucoviscidose Adultes, Service de Pneumologie, Centre Hospitalier Universitaire, Angers</wicri:regionArea><placeName><settlement type="city">Angers</settlement></placeName></affiliation></author><author><name sortKey="Troussier, Francoise" sort="Troussier, Francoise" uniqKey="Troussier F" first="Françoise" last="Troussier">Françoise Troussier</name><affiliation wicri:level="1"><nlm:affiliation>Centre de Ressources et de Compétences en Mucoviscidose Enfants, Service de Pédiatrie, Centre Hospitalier Universitaire, Angers, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Centre de Ressources et de Compétences en Mucoviscidose Enfants, Service de Pédiatrie, Centre Hospitalier Universitaire, Angers</wicri:regionArea><placeName><settlement type="city">Angers</settlement></placeName></affiliation></author><author><name sortKey="Ballet, Jean Jacques" sort="Ballet, Jean Jacques" uniqKey="Ballet J" first="Jean Jacques" last="Ballet">Jean Jacques Ballet</name><affiliation wicri:level="1"><nlm:affiliation>Laboratoire de Parasitologie-Mycologie, Centre Hospitalier Universitaire, Rouen, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Parasitologie-Mycologie, Centre Hospitalier Universitaire, Rouen</wicri:regionArea><placeName><settlement type="city">Rouen</settlement></placeName></affiliation></author><author><name sortKey="Gargala, Gilles" sort="Gargala, Gilles" uniqKey="Gargala G" first="Gilles" last="Gargala">Gilles Gargala</name><affiliation wicri:level="3"><nlm:affiliation>Laboratoire de Parasitologie-Mycologie, Centre Hospitalier Universitaire, Rouen, France Normandie Univ, UNIROUEN, EA 3800, 76000 Rouen, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Parasitologie-Mycologie, Centre Hospitalier Universitaire, Rouen, France Normandie Univ, UNIROUEN, EA 3800, 76000 Rouen</wicri:regionArea><placeName><region type="region" nuts="2">Région Normandie</region><region type="old region" nuts="2">Haute-Normandie</region><settlement type="city">Rouen</settlement></placeName></affiliation></author><author><name sortKey="Zouhair, Rachid" sort="Zouhair, Rachid" uniqKey="Zouhair R" first="Rachid" last="Zouhair">Rachid Zouhair</name><affiliation wicri:level="1"><nlm:affiliation>University Moulay Ismail, Faculty of Sciences, Meknes, Morocco.</nlm:affiliation><country xml:lang="fr">Maroc</country><wicri:regionArea>University Moulay Ismail, Faculty of Sciences, Meknes</wicri:regionArea><wicri:noRegion>Meknes</wicri:noRegion></affiliation></author><author><name sortKey="Bougnoux, Marie Elisabeth" sort="Bougnoux, Marie Elisabeth" uniqKey="Bougnoux M" first="Marie-Elisabeth" last="Bougnoux">Marie-Elisabeth Bougnoux</name><affiliation wicri:level="1"><nlm:affiliation>Laboratoire de Parasitologie-Mycologie, Centre Hospitalier Universitaire Necker-Enfants Malades, Paris, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Parasitologie-Mycologie, Centre Hospitalier Universitaire Necker-Enfants Malades, Paris</wicri:regionArea><placeName><settlement type="city">Paris</settlement></placeName></affiliation></author><author><name sortKey="Bouchara, Jean Philippe" sort="Bouchara, Jean Philippe" uniqKey="Bouchara J" first="Jean-Philippe" last="Bouchara">Jean-Philippe Bouchara</name><affiliation wicri:level="1"><nlm:affiliation>L'UNAM Université, Université d'Angers, Groupe d'Etude des Interactions Hôte-Pathogène, EA 3142, Angers, France Laboratoire de Parasitologie-Mycologie, Centre Hospitalier Universitaire, Angers, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>L'UNAM Université, Université d'Angers, Groupe d'Etude des Interactions Hôte-Pathogène, EA 3142, Angers, France Laboratoire de Parasitologie-Mycologie, Centre Hospitalier Universitaire, Angers</wicri:regionArea><placeName><settlement type="city">Angers</settlement></placeName></affiliation></author><author><name sortKey="Favennec, Loic" sort="Favennec, Loic" uniqKey="Favennec L" first="Loïc" last="Favennec">Loïc Favennec</name><affiliation wicri:level="3"><nlm:affiliation>Laboratoire de Parasitologie-Mycologie, Centre Hospitalier Universitaire, Rouen, France Normandie Univ, UNIROUEN, EA 3800, 76000 Rouen, France loic.favennec@chu-rouen.fr.</nlm:affiliation><country wicri:rule="url">France</country><wicri:regionArea>Laboratoire de Parasitologie-Mycologie, Centre Hospitalier Universitaire, Rouen, France Normandie Univ, UNIROUEN, EA 3800, 76000 Rouen</wicri:regionArea><placeName><region type="region" nuts="2">Région Normandie</region><region type="old region" nuts="2">Haute-Normandie</region><settlement type="city">Rouen</settlement></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2016">2016</date><idno type="RBID">pubmed:27605712</idno><idno type="pmid">27605712</idno><idno type="doi">10.1128/JCM.01462-16</idno><idno type="wicri:Area/PubMed/Corpus">000015</idno><idno type="wicri:Area/PubMed/Curation">000015</idno><idno type="wicri:Area/PubMed/Checkpoint">000015</idno><idno type="wicri:Area/Ncbi/Merge">000565</idno><idno type="wicri:Area/Ncbi/Curation">000565</idno><idno type="wicri:Area/Ncbi/Checkpoint">000565</idno><idno type="wicri:Area/Main/Merge">000050</idno><idno type="wicri:Area/Main/Curation">000051</idno><idno type="wicri:Area/Main/Exploration">000051</idno><idno type="wicri:Area/France/Extraction">000050</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Long-term Rasamsonia argillacea complex species colonization revealed by rep-PCR in cystic fibrosis patients.</title><author><name sortKey="Mouhajir, Abdelmounaim" sort="Mouhajir, Abdelmounaim" uniqKey="Mouhajir A" first="Abdelmounaim" last="Mouhajir">Abdelmounaim Mouhajir</name><affiliation wicri:level="1"><nlm:affiliation>L'UNAM Université, Université d'Angers, Groupe d'Etude des Interactions Hôte-Pathogène, EA 3142, Angers, France University Moulay Ismail, Faculty of Sciences, Meknes, Morocco.</nlm:affiliation><country xml:lang="fr">Maroc</country><wicri:regionArea>L'UNAM Université, Université d'Angers, Groupe d'Etude des Interactions Hôte-Pathogène, EA 3142, Angers, France University Moulay Ismail, Faculty of Sciences, Meknes</wicri:regionArea><wicri:noRegion>Meknes</wicri:noRegion></affiliation></author><author><name sortKey="Matray, Olivier" sort="Matray, Olivier" uniqKey="Matray O" first="Olivier" last="Matray">Olivier Matray</name><affiliation wicri:level="3"><nlm:affiliation>Laboratoire de Parasitologie-Mycologie, Centre Hospitalier Universitaire, Rouen, France Normandie Univ, UNIROUEN, EA 3800, 76000 Rouen, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Parasitologie-Mycologie, Centre Hospitalier Universitaire, Rouen, France Normandie Univ, UNIROUEN, EA 3800, 76000 Rouen</wicri:regionArea><placeName><region type="region" nuts="2">Région Normandie</region><region type="old region" nuts="2">Haute-Normandie</region><settlement type="city">Rouen</settlement></placeName></affiliation></author><author><name sortKey="Giraud, Sandrine" sort="Giraud, Sandrine" uniqKey="Giraud S" first="Sandrine" last="Giraud">Sandrine Giraud</name><affiliation wicri:level="1"><nlm:affiliation>L'UNAM Université, Université d'Angers, Groupe d'Etude des Interactions Hôte-Pathogène, EA 3142, Angers, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>L'UNAM Université, Université d'Angers, Groupe d'Etude des Interactions Hôte-Pathogène, EA 3142, Angers</wicri:regionArea><placeName><settlement type="city">Angers</settlement></placeName></affiliation></author><author><name sortKey="Mely, Laurent" sort="Mely, Laurent" uniqKey="Mely L" first="Laurent" last="Mély">Laurent Mély</name><affiliation wicri:level="1"><nlm:affiliation>Centre de Ressources et de Compétences en Mucoviscidose, Hôpital Renée Sabran, Giens, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Centre de Ressources et de Compétences en Mucoviscidose, Hôpital Renée Sabran, Giens</wicri:regionArea><wicri:noRegion>Giens</wicri:noRegion><wicri:noRegion>Giens</wicri:noRegion></affiliation></author><author><name sortKey="Marguet, Christophe" sort="Marguet, Christophe" uniqKey="Marguet C" first="Christophe" last="Marguet">Christophe Marguet</name><affiliation wicri:level="1"><nlm:affiliation>Centre de Ressources et de Compétences en Mucoviscidose, Service de Pédiatrie, Hôpital Charles Nicolle, Rouen, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Centre de Ressources et de Compétences en Mucoviscidose, Service de Pédiatrie, Hôpital Charles Nicolle, Rouen</wicri:regionArea><placeName><settlement type="city">Rouen</settlement></placeName></affiliation></author><author><name sortKey="Sermet Gaudelus, Isabelle" sort="Sermet Gaudelus, Isabelle" uniqKey="Sermet Gaudelus I" first="Isabelle" last="Sermet-Gaudelus">Isabelle Sermet-Gaudelus</name><affiliation wicri:level="1"><nlm:affiliation>Centre de Ressources et de Compétences en Mucoviscidose, Unité de Pédiatrie, Hôpital Necker-Enfants Malades, Paris, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Centre de Ressources et de Compétences en Mucoviscidose, Unité de Pédiatrie, Hôpital Necker-Enfants Malades, Paris</wicri:regionArea><placeName><settlement type="city">Paris</settlement></placeName></affiliation></author><author><name sortKey="Le Gal, Solene" sort="Le Gal, Solene" uniqKey="Le Gal S" first="Solène" last="Le Gal">Solène Le Gal</name><affiliation wicri:level="1"><nlm:affiliation>L'UNAM Université, Université d'Angers, Groupe d'Etude des Interactions Hôte-Pathogène, EA 3142, Angers, France Laboratoire de Parasitologie-Mycologie, Centre Hospitalier Universitaire, Brest, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>L'UNAM Université, Université d'Angers, Groupe d'Etude des Interactions Hôte-Pathogène, EA 3142, Angers, France Laboratoire de Parasitologie-Mycologie, Centre Hospitalier Universitaire, Brest</wicri:regionArea><placeName><settlement type="city">Brest</settlement></placeName></affiliation></author><author><name sortKey="Labbe, Franck" sort="Labbe, Franck" uniqKey="Labbe F" first="Franck" last="Labbé">Franck Labbé</name><affiliation wicri:level="1"><nlm:affiliation>Laboratoire de Microbiologie, Hôpital Jacques Monod, Le Havre, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Microbiologie, Hôpital Jacques Monod, Le Havre</wicri:regionArea><placeName><settlement type="city">Le Havre</settlement></placeName></affiliation></author><author><name sortKey="Person, Christine" sort="Person, Christine" uniqKey="Person C" first="Christine" last="Person">Christine Person</name><affiliation wicri:level="1"><nlm:affiliation>Centre de Ressources et de Compétences en Mucoviscidose Adultes, Service de Pneumologie, Centre Hospitalier Universitaire, Angers, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Centre de Ressources et de Compétences en Mucoviscidose Adultes, Service de Pneumologie, Centre Hospitalier Universitaire, Angers</wicri:regionArea><placeName><settlement type="city">Angers</settlement></placeName></affiliation></author><author><name sortKey="Troussier, Francoise" sort="Troussier, Francoise" uniqKey="Troussier F" first="Françoise" last="Troussier">Françoise Troussier</name><affiliation wicri:level="1"><nlm:affiliation>Centre de Ressources et de Compétences en Mucoviscidose Enfants, Service de Pédiatrie, Centre Hospitalier Universitaire, Angers, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Centre de Ressources et de Compétences en Mucoviscidose Enfants, Service de Pédiatrie, Centre Hospitalier Universitaire, Angers</wicri:regionArea><placeName><settlement type="city">Angers</settlement></placeName></affiliation></author><author><name sortKey="Ballet, Jean Jacques" sort="Ballet, Jean Jacques" uniqKey="Ballet J" first="Jean Jacques" last="Ballet">Jean Jacques Ballet</name><affiliation wicri:level="1"><nlm:affiliation>Laboratoire de Parasitologie-Mycologie, Centre Hospitalier Universitaire, Rouen, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Parasitologie-Mycologie, Centre Hospitalier Universitaire, Rouen</wicri:regionArea><placeName><settlement type="city">Rouen</settlement></placeName></affiliation></author><author><name sortKey="Gargala, Gilles" sort="Gargala, Gilles" uniqKey="Gargala G" first="Gilles" last="Gargala">Gilles Gargala</name><affiliation wicri:level="3"><nlm:affiliation>Laboratoire de Parasitologie-Mycologie, Centre Hospitalier Universitaire, Rouen, France Normandie Univ, UNIROUEN, EA 3800, 76000 Rouen, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Parasitologie-Mycologie, Centre Hospitalier Universitaire, Rouen, France Normandie Univ, UNIROUEN, EA 3800, 76000 Rouen</wicri:regionArea><placeName><region type="region" nuts="2">Région Normandie</region><region type="old region" nuts="2">Haute-Normandie</region><settlement type="city">Rouen</settlement></placeName></affiliation></author><author><name sortKey="Zouhair, Rachid" sort="Zouhair, Rachid" uniqKey="Zouhair R" first="Rachid" last="Zouhair">Rachid Zouhair</name><affiliation wicri:level="1"><nlm:affiliation>University Moulay Ismail, Faculty of Sciences, Meknes, Morocco.</nlm:affiliation><country xml:lang="fr">Maroc</country><wicri:regionArea>University Moulay Ismail, Faculty of Sciences, Meknes</wicri:regionArea><wicri:noRegion>Meknes</wicri:noRegion></affiliation></author><author><name sortKey="Bougnoux, Marie Elisabeth" sort="Bougnoux, Marie Elisabeth" uniqKey="Bougnoux M" first="Marie-Elisabeth" last="Bougnoux">Marie-Elisabeth Bougnoux</name><affiliation wicri:level="1"><nlm:affiliation>Laboratoire de Parasitologie-Mycologie, Centre Hospitalier Universitaire Necker-Enfants Malades, Paris, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Parasitologie-Mycologie, Centre Hospitalier Universitaire Necker-Enfants Malades, Paris</wicri:regionArea><placeName><settlement type="city">Paris</settlement></placeName></affiliation></author><author><name sortKey="Bouchara, Jean Philippe" sort="Bouchara, Jean Philippe" uniqKey="Bouchara J" first="Jean-Philippe" last="Bouchara">Jean-Philippe Bouchara</name><affiliation wicri:level="1"><nlm:affiliation>L'UNAM Université, Université d'Angers, Groupe d'Etude des Interactions Hôte-Pathogène, EA 3142, Angers, France Laboratoire de Parasitologie-Mycologie, Centre Hospitalier Universitaire, Angers, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>L'UNAM Université, Université d'Angers, Groupe d'Etude des Interactions Hôte-Pathogène, EA 3142, Angers, France Laboratoire de Parasitologie-Mycologie, Centre Hospitalier Universitaire, Angers</wicri:regionArea><placeName><settlement type="city">Angers</settlement></placeName></affiliation></author><author><name sortKey="Favennec, Loic" sort="Favennec, Loic" uniqKey="Favennec L" first="Loïc" last="Favennec">Loïc Favennec</name><affiliation wicri:level="3"><nlm:affiliation>Laboratoire de Parasitologie-Mycologie, Centre Hospitalier Universitaire, Rouen, France Normandie Univ, UNIROUEN, EA 3800, 76000 Rouen, France loic.favennec@chu-rouen.fr.</nlm:affiliation><country wicri:rule="url">France</country><wicri:regionArea>Laboratoire de Parasitologie-Mycologie, Centre Hospitalier Universitaire, Rouen, France Normandie Univ, UNIROUEN, EA 3800, 76000 Rouen</wicri:regionArea><placeName><region type="region" nuts="2">Région Normandie</region><region type="old region" nuts="2">Haute-Normandie</region><settlement type="city">Rouen</settlement></placeName></affiliation></author></analytic><series><title level="j">Journal of clinical microbiology</title><idno type="eISSN">1098-660X</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The aim of this work was to document molecular epidemiology of Rasamsonia argillacea species complex isolates from cystic fibrosis (CF) patients. In this work, 116 isolates belonging to this species complex and collected from 26 CF patients and one patient with chronic granulomatous disease were characterized using PCR amplification assays of repetitive DNA sequences, and electrophoretic separation of amplicons (rep-PCR). Data revealed a clustering consistent with molecular species identification. A single species was recovered from most patients. Rasamsonia aegroticola was the most common species, followed by R. argillacea stricto sensu and R. piperina, while R. eburnea was not identified. Of 29 genotypes, 7 were shared by distinct patients, while 22 were patient-specific. In each clinical sample, most isolates exhibited an identical genotype. Genotyping of isolates recovered from sequential samples from the same patient confirmed the capability of R. aegroticola and R. argillacea isolates to chronically colonize the airways. A unique genotype was recovered from two siblings during a 6-month period. In the other cases, a largely dominant genotype was detected. Present results which support the use of rep-PCR for both identification and genotyping for the R. argillacea species complex, provide the first molecular evidence of chronic airway colonization by these fungi in CF patients.</div></front></TEI><affiliations><list><country><li>France</li><li>Maroc</li></country><region><li>Haute-Normandie</li><li>Région Normandie</li></region><settlement><li>Angers</li><li>Brest</li><li>Le Havre</li><li>Paris</li><li>Rouen</li></settlement></list><tree><country name="Maroc"><noRegion><name sortKey="Mouhajir, Abdelmounaim" sort="Mouhajir, Abdelmounaim" uniqKey="Mouhajir A" first="Abdelmounaim" last="Mouhajir">Abdelmounaim Mouhajir</name></noRegion><name sortKey="Zouhair, Rachid" sort="Zouhair, Rachid" uniqKey="Zouhair R" first="Rachid" last="Zouhair">Rachid Zouhair</name></country><country name="France"><region name="Région Normandie"><name sortKey="Matray, Olivier" sort="Matray, Olivier" uniqKey="Matray O" first="Olivier" last="Matray">Olivier Matray</name></region><name sortKey="Ballet, Jean Jacques" sort="Ballet, Jean Jacques" uniqKey="Ballet J" first="Jean Jacques" last="Ballet">Jean Jacques Ballet</name><name sortKey="Bouchara, Jean Philippe" sort="Bouchara, Jean Philippe" uniqKey="Bouchara J" first="Jean-Philippe" last="Bouchara">Jean-Philippe Bouchara</name><name sortKey="Bougnoux, Marie Elisabeth" sort="Bougnoux, Marie Elisabeth" uniqKey="Bougnoux M" first="Marie-Elisabeth" last="Bougnoux">Marie-Elisabeth Bougnoux</name><name sortKey="Favennec, Loic" sort="Favennec, Loic" uniqKey="Favennec L" first="Loïc" last="Favennec">Loïc Favennec</name><name sortKey="Gargala, Gilles" sort="Gargala, Gilles" uniqKey="Gargala G" first="Gilles" last="Gargala">Gilles Gargala</name><name sortKey="Giraud, Sandrine" sort="Giraud, Sandrine" uniqKey="Giraud S" first="Sandrine" last="Giraud">Sandrine Giraud</name><name sortKey="Labbe, Franck" sort="Labbe, Franck" uniqKey="Labbe F" first="Franck" last="Labbé">Franck Labbé</name><name sortKey="Le Gal, Solene" sort="Le Gal, Solene" uniqKey="Le Gal S" first="Solène" last="Le Gal">Solène Le Gal</name><name sortKey="Marguet, Christophe" sort="Marguet, Christophe" uniqKey="Marguet C" first="Christophe" last="Marguet">Christophe Marguet</name><name sortKey="Mely, Laurent" sort="Mely, Laurent" uniqKey="Mely L" first="Laurent" last="Mély">Laurent Mély</name><name sortKey="Person, Christine" sort="Person, Christine" uniqKey="Person C" first="Christine" last="Person">Christine Person</name><name sortKey="Sermet Gaudelus, Isabelle" sort="Sermet Gaudelus, Isabelle" uniqKey="Sermet Gaudelus I" first="Isabelle" last="Sermet-Gaudelus">Isabelle Sermet-Gaudelus</name><name sortKey="Troussier, Francoise" sort="Troussier, Francoise" uniqKey="Troussier F" first="Françoise" last="Troussier">Françoise Troussier</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/France/explor/LeHavreV1/Data/France/Analysis
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000050 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/France/Analysis/biblio.hfd -nk 000050 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/France
|area= LeHavreV1
|flux= France
|étape= Analysis
|type= RBID
|clé= pubmed:27605712
|texte= Long-term Rasamsonia argillacea complex species colonization revealed by rep-PCR in cystic fibrosis patients.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/France/Analysis/RBID.i -Sk "pubmed:27605712" \
| HfdSelect -Kh $EXPLOR_AREA/Data/France/Analysis/biblio.hfd \
| NlmPubMed2Wicri -a LeHavreV1
| This area was generated with Dilib version V0.6.25. |  |